Journal: Molecules

Article Title: (2E,5E)-2,5-Bis(3-hydroxy-4-methoxybenzylidene) cyclopentanone Exerts Anti-Melanogenesis and Anti-Wrinkle Activities in B16F10 Melanoma and Hs27 Fibroblast Cells

doi: 10.3390/molecules23061415

Figure Lengend Snippet: Figure 3. Cell viability of BHCP on B16F10 melanoma (a) and Hs27 fibroblast cells (b). Cells were treated with various concentrations (0–10 µM) of BHCP for 24 h and 48 h, respectively, and cell viability was measured by EZ-Cytox assay.

Article Snippet: The human skin fibroblast cell line Hs27 was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Molecules

Article Title: (2E,5E)-2,5-Bis(3-hydroxy-4-methoxybenzylidene) cyclopentanone Exerts Anti-Melanogenesis and Anti-Wrinkle Activities in B16F10 Melanoma and Hs27 Fibroblast Cells

doi: 10.3390/molecules23061415

Figure Lengend Snippet: Figure 6. Effects of BHCP on the expression levels of p-p65 (Ser536) in UV-induced Hs27 human fibroblast cells. Cells were preincubated with or without 1 and 10 µM BHCP for 24 h and were stimulated with UV (50 mJ/cm2) for an additional 24 h. Nuclear protein levels of p-p65 (Ser536) were determined by Western blotting (a); After normalization to TFIIB, relative ratios were quantitated with CS analyzer software (b); Cytosol protein levels of p65 was determined by Western blotting (c); After normalization to β-actin, ratios were quantitated with CS analyzer software (d). Data are expressed as mean ± S.E.M. of three independent experiments. # p < 0.05 versus untreated control; * p < 0.05 and ** p < 0.01 versus α-MSH stimulation.

Article Snippet: The human skin fibroblast cell line Hs27 was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Western Blot, Software, Control

Journal: Molecules

Article Title: (2E,5E)-2,5-Bis(3-hydroxy-4-methoxybenzylidene) cyclopentanone Exerts Anti-Melanogenesis and Anti-Wrinkle Activities in B16F10 Melanoma and Hs27 Fibroblast Cells

doi: 10.3390/molecules23061415

Figure Lengend Snippet: Figure 7. Effects of BHCP on the expression levels of MMP-1, MMP-9, MMP-12, and MMP-13 in UV-induced Hs27 human fibroblast cells. Cells were preincubated with or without 1 and 10 µM BHCP for 24 h and were stimulated with UV (50 mJ/cm2) for an additional 24 h. Harvested cells were lysed and then examined for the expression levels of MMP-1, MMP-9, MMP-12, and MMP-13 by Western blotting (a); The protein levels of MMP-1 (b); MMP-9 (c); MMP-12 (d); and MMP-13 (e) were quantitated with CS analyzer software. MMP-1, MMP-9, MMP-12, and MMP-13 protein levels were normalized by β-actin. Data are expressed as mean ± S.E.M. of three independent experiments. # p < 0.05 versus untreated control; * p < 0.05 versus UV stimulation.

Article Snippet: The human skin fibroblast cell line Hs27 was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Western Blot, Software, Control

Journal: Nature immunology

Article Title: Alarmin S100A11 initiates a chemokine response to the human pathogen Toxoplasma gondii.

doi: 10.1038/s41590-018-0250-8

Figure Lengend Snippet: Fig. 5 | Role of caspase-1 in S100A11 release. a,b, Analysis of S100A11 protein levels in Pru- or RH88-infected fibroblasts by immunofluorescent detection (a) and by expression analysis (b). Scale bars, 50 μm. Error bars represent mean ± s.d. c, CCL2 expression by THP-1 cells stimulated with cell culture supernatants collected from frozen cells in comparison with RH88-infected cells. d, Analysis of caspase-1 activity in Hs27 and THP-1 cells infected with RH88 and Pru strains of T. gondii. CCL2 expression in THP-1 cells stimulated with cell culture supernatants collected from human Hs27 fibroblasts infected with RH88 alone or in the presence of zVAD (10 μM) or zWEHD (10 μM) for 72 h was measured by quantitative rtPCR. e, Detection of S1000A11 in cell culture supernatants collected from Hs27 cells, Hs27 cells infected with RH88 (Hs27 + RH; MOI, 3:1) or Pru (Hs27 + Pru; MOI, 3:1), Hs27 cells killed by rapid freezing (frozen Hs27), or Hs27 cells infected with RH88 in the presence of zVAD (Hs27 + RH + zVAD). Hs27 + RH + dimethylsulfoxide (DMSO) represents an additional vehicle control supernatant for the zVAD-treated cells. Each symbol represents an individual experimental sample, n = 3 or n = 4 (b–d). The data shown are representative of three (a–d) and five (e) independent experiments, and error bars represent mean ± s.d.

Article Snippet: THP-1 cells (TIB-202) and Hs27 fibroblasts (RL-1634) were purchased from American Type Culture Collection.

Techniques: Infection, Expressing, Cell Culture, Comparison, Activity Assay, Reverse Transcription Polymerase Chain Reaction, Control

Journal: International Journal of Molecular Sciences

Article Title: 4-Arylthiosemicarbazide Derivatives as Toxoplasmic Aromatic Amino Acid Hydroxylase Inhibitors and Anti-inflammatory Agents

doi: 10.3390/ijms23063213

Figure Lengend Snippet: The inhibitory concentrations (IC 50 ) against Toxoplasma gondii and cytotoxic concentrations (CC 30 ) against L929 and Hs27 cell lines of 2a and 6a .

Article Snippet: Hs27 cells (human fibroblast, ATCC ® CRL-1634TM, Manassas, VA, USA) were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, ATCC ® 30-2002TM) supplemented with 10% fetal bovine serum (FBS, ATCC ® 30-2020TM), 100 IU/mL penicillin and 100 μg/mL streptomycin (ATCC ® 30-2300TM).

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